Device and Method for Detecting Antibiotic Inactivating Enzymes

No Thumbnail Available
Authors
Black, Jennifer A.
Moland, Ellen Smith
Thomson, Kenneth S.
Issue Date
2007-01-23
Type
Patent
Language
Keywords
Research Projects
Organizational Units
Journal Issue
Alternative Title
Abstract
The present invention provides a method of determining the antibiotic susceptibility of a microorganism comprising the following steps. First, a culture of the microorganism whose susceptibility is to be determined is admixed with an antibiotic to which susceptibility is to be assayed, and a per- meabilizing agent for the microorganism present in a non- growth-inhibiting microorganism-permeabilizing effective amount to form an assay culture. Next, the assay culture is incubated under appropriate culture conditions and for a time sufficient to determine the susceptibility of the microorganism to the antibiotic. In another aspect, the present invention provides an improved method for antibiotic susceptibility testing of a microorganism in a culture by admixing the culture with an antibiotic to which susceptibility is to be assayed, and incubating the culture for a time sufficient to determine the susceptibility of the microorganism to the antibiotic, the improvement comprising admixing the culture with a permeabilizing agent for the microorganism present in a non-growth inhibiting microorganism-permeabilizing effective amount.
Description
Citation
Publisher
United States Patent Office
License
Journal
Volume
Issue
PubMed ID
DOI
ISSN
EISSN