Device and Direct Method for Detection of Antibiotic-Inactivating Enzymes
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Authors
Black, Jennifer A.
Thomson, Kenneth S.
Issue Date
2010-10-05
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Type
Patent
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Abstract
A method for determining whether a microorganism produces an AmpC B-lactamase is disclosed in which a culture of a microorganism suspected of producing a B-lactamase that inactivates a B-lactam-containing antibiotic is admixed with an effective amount of each of i) a B-lactam-containing antibiotic, ii) a B-lactamase inhibitorto whichAmpC B-lactamase is resistant, and iii) a permeabilizing agent for the microorganism present in a non-growth-inhibiting microorganism- permeabilizing amount to form an assay culture. That assay culture in maintained under appropriate culture conditions and for a time period sufficient to determine the interaction of the microorganism with the AmpC B-lactamase resistant inhibitor and antibacterial compound, and thereby determine the presence of an AmpC B-lactamase, wherein a positive test indicates the presence of an AmpC B-lactamase.
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Publisher
United States Patent Office