A Comparison of Growth of Primary Mouse Marrow Explants
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Authors
Wallace, Judith Ann
Issue Date
1965
Volume
Issue
Type
Thesis
Language
en_US
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Abstract
The growth of cells in vitro is a technique which presents many advantages for use in biological research, particularly in the fields of genetics, physiology, and biochemistry. It is possible to follow the metabolic and chemical actions of the cell in a controlled manner since an isolated population of cells is used for study. This degree of environmental control has made tissue culture an ideal technique for biochemical and cytological studies. | Many of the problems encountered in research require the growth of a single, isolated cell into a clonal population. For example, a problem dealing with genetics would require working with a cell population having the same genetic expression in each cell. This can be accomplished by developing the population from one single, isolated cell. | Success ingrowing a single, isolated cell in tissue culture has been limited. Small populations of cells have been successfully maintained and grown using chemically defined media and media enriched with non-defined constituents such as horse serum and chick embryo extract. However the media which are presently available are inadequate for the support and proliferation of any single, isolated cell of primary explant.
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Citation
Publisher
Creighton University
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A non-exclusive distribution right is granted to Creighton University and to ProQuest following the publishing model selected above.