Device and Direct Method for Detecting Antibiotic-Inactivating Factors
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Authors
Black, Jennifer A.
Moland, Ellen Smith
Thomson, Kenneth S.
Issue Date
2010-05-18
Type
Patent
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Abstract
The present invention provides a method of determining the antibiotic susceptibility of a microorganism comprising the following steps. First, a culture of the microorganism whose susceptibility is to be determined is admixed with an antibiotic to which susceptibility is to be assayed, and a permeabi- lizing agent for the microorganism present in a non-growth- inhibiting microorganism-permeabilizing effective amount to form an assay culture. Next, the assay culture is incubated under appropriate culture conditions and for a time sufficient to determine the susceptibility of the microorganism to the antibiotic. In another aspect, the present invention provides an improved method for antibiotic susceptibility testing of a microorganism in a culture by admixing the culture with an antibiotic to which susceptibility is to be assayed, and incubating the culture for a time sufficient to determine the susceptibility of the microorganism to the antibiotic, the improvement comprising admixing the culture with a perme- abilizing agent for the microorganism present in a nongrowth inhibiting microorganism-permeabilizing effective amount.
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Publisher
United States Patent Office