Device and Direct Method for Detecting Antibiotic-Inactivating Factors

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Authors

Black, Jennifer A.
Moland, Ellen Smith
Thomson, Kenneth S.

Issue Date

2010-05-18

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Patent

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Abstract

The present invention provides a method of determining the antibiotic susceptibility of a microorganism comprising the following steps. First, a culture of the microorganism whose susceptibility is to be determined is admixed with an antibiotic to which susceptibility is to be assayed, and a permeabi- lizing agent for the microorganism present in a non-growth- inhibiting microorganism-permeabilizing effective amount to form an assay culture. Next, the assay culture is incubated under appropriate culture conditions and for a time sufficient to determine the susceptibility of the microorganism to the antibiotic. In another aspect, the present invention provides an improved method for antibiotic susceptibility testing of a microorganism in a culture by admixing the culture with an antibiotic to which susceptibility is to be assayed, and incubating the culture for a time sufficient to determine the susceptibility of the microorganism to the antibiotic, the improvement comprising admixing the culture with a perme- abilizing agent for the microorganism present in a nongrowth inhibiting microorganism-permeabilizing effective amount.

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United States Patent Office

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