Method of Gene Transfer Using Retrotransposons
Loading...
Authors
Hodgson, Clague P.
Issue Date
1994-10-11
Volume
Issue
Type
Patent
Language
Keywords
Alternative Title
Abstract
A process for the transfer and/or expression of any gene into cells, organisms or species is disclosed. The process involves isolating the gene, introducing the gene into a vector comprised of at least one retrotransposon genetic element to provide a hybrid gene. The hybrid gene is introduced into a donor cell capable of packaging and transmitting the retrotransposon to other cells, organisms or species. The hybrid gene is transferred to a recipient cell wherein the hybrid gene replicates by reverse transcription and is inserted into the recipient cell’s genome. The gene is preferably expressed as RNA and/or protein, giving the phenotype of the gene.
Description
Citation
Publisher
United States Patent Office